Johns Hopkins, founded in 1876, is America's first research university and home to nine world-class academic divisions working together as one university.
We are seeking a Research Specialist II to join the lab at Johns Hopkins All Children's Hospital in St. Petersburg, Florida. This position will work in a lab focused on cancer immunology research investigating the tumor microenvironment and anti-tumor immune responses, including organoid and primary cell culture systems and in vivo preclinical tumor models. The specialist will contribute to immunotherapy and translational research studies within a collaborative and dynamic laboratory environment.
The Research Specialist II develops assays and protocols for PI approval and designs experimental approaches for laboratory projects. Performs a range of sophisticated assays. Makes significant contributions to various aspects of laboratory efforts and goals. Responsible for troubleshooting, independent decisions and training/guidance of other laboratory personnel.
Specific Duties & Responsibilities
- Conduct assigned laboratory projects.
- Utilize specialized knowledge to interpret results, e.g. read controls, evaluate results, etc.
- Work independently and use scientific knowledge and lab experience to make judgments and decisions.
- Teach/train learners, and new technicians in the laboratory.
- Guide, direct, and facilitate problem solving for entry-level laboratory members on day-to-day problems with more challenging research assays.
- Make scientific contributions to protocol development, efficiency, and productivity.
- Collaborate in the development of new techniques and instruments.
- Apply published research methods to current experimental work by investigating published protocols and discussing them with PI.
- Scientifically contribute to the preparation of manuscripts and proposals.
- Process analyzed data into figures for scientific publications.
- Order equipment and supplies, interface with vendors.
- Maintain daily records of experiments.
- Organize and track laboratory samples.
- Other duties as assigned.
Minimum Qualifications
- Bachelor's Degree in Biology, Chemistry or related field.
- Two years of laboratory experience performing relevant techniques including experimental techniques used in genomics, sequencing, and cancer biology assays.
- Additional education may substitute for required experience and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
- Master's Degree with related graduate research.
- Cell culture.
- RNA isolation and quantitative PCR (qPCR).
- Common cell biology assays.
- Genomics and sequencing.
- Immunostaining.
- Western blotting.
- FACS analysis.
- Mouse handling.
- Bioinformatics analysis.
- GraphPad/Prism/Adobe.
Technical Skills & Expected Level of Proficiency
- Assay Development and Validation - Developing
- Data Management and Analysis - Developing
- Experimental Design and Execution - Developing
- Operations Management - Developing
- Protocol Development and Optimization - Developing
- Scientific Writing - Developing
- Stakeholder Engagement - Developing
The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs.
Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $42,330 - $74,110 Annually ($55,650 targeted; Commensurate w/exp.)
Employee group: Full Time
Schedule: Mon-Fri, 8.30am-5.00pm
FLSA Status: Exempt
Location: Florida
Department name: All Childrens
Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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