Research Associate – Molecular Biologist - JOBS.ac.kr(잡스) - 대한민국 최대 석박사 채용정보, 잡스!

{"job":{"jobId":2422867,"fk_accountId":null,"fk_organizationId":494,"fk_adminAccountId":null,"title":"Research Associate – Molecular Biologist","description":"\n\nJob description\n\n\nThe Comprehensive Cancer Centre (CCC) at King’s College London (KCL) is looking for two highly enthusiastic research scientists to drive collaborative research projects in the laboratory of Prof. Tony Ng, Head of the CCC. \n\n\n \n\n\nThe postholder will be part of the GlaxoSmithKline(GSK)-KCL Translational Oncology Research Hub, an exclusive research collaboration between GSK and the CCC at KCL. This partnership aims to create a personalised strategy for patients at high risk of recurrence by monitoring for dynamic biomarkers that can predict resistance during treatment or recurrence in the post-treatment phase (  <a data-mz=\"\" href=\"https://www.theguardian.com/business/2021/sep/17/gsk-teams-with-kings-college-to-use-ai-to-fight-cancer?bingParse\" rel=\"no-follow\">https://www.theguardian.com/business/2021/sep/17/gsk-teams-with-kings-college-to-use-ai-to-fight-cancer?bingParse</a>).  \n\n\n \n\n\nThe team will use patient derived organoids (PDOs) and state-of-the-art imaging techniques to study how tumour cells interact with immune cells. These will be modelled in collaboration with the GSK Artificial Intelligence Unit to distinguish between high and low risk patients.\n\n\n \n\n\nThe postholder will be responsible for developing and maintaining PDOs and will apply a number of molecular and cell biology and imaging assays in order to identify and model the tumour genomic, immune and exosomal changes that will distinguish patients that will have high and low risk of recurrence after primary treatment.\n\n\n \n\n\nScientifically, we have recently demonstrated several tumour-immune crosstalk at the levels of both transcriptional reprogramming and protein post-translational modifications. These crosstalk mechanisms are shown to have wide biological and therapeutic implications (succinate dehydrogenase downregulation leading to HIF up-regulation, induction of TGFb, upregulation of the DAMP molecule S100A9 in the premetastatic niche, activation of STING in macrophages and resistance to EGFR therapies etc) secondary to heterotypic cell-cell contacts made between tumour cells and macrophages (Eisenblaetter, Theranostics 7: 2392-2401, 2017; Evans, Cell Rep 27: 1967-1978, 2019; Gomez, Science Signaling 13, 2020; Vicencio, Evans et al., Cell Death and Disease, in press; Weitsman, Oncogene 36: 3618-3628, 2017). These genomic and proteomic changes will be further explored in the context of the Research Hub. \n\n\n \n\n\nThere are two positions available. Both posts will be offered on a full-time, fixed term contract for 3 years.\n\n\n \n\n\n \n\n\nKey responsibilities\n\n\nSpecific  \n\n\n<ul>\n<li>To generate and maintain patient derived organoids (PDOs) from breast, lung, colorectal, head and neck cancer tissue samples  </li>\n<li>To treat PDOs with clinically relevant drugs and to perform downstream assays to examine changes in organoid gene expression and chromatin structure/integrity.     </li>\n<li>To isolate exosomes from PDO culture supernatants and to perform downstream assays to examine the protein and nucleic acid content of these vesicles. </li>\n<li>To work with others to use multiphoton and/or lightsheet imaging of tumour cells interacting with immune cells in organoids, to identify and model the tumour genomic, immune and exosomal changes. </li>\n<li>To maintain clear and detailed records of all experimental work undertaken in accordance with the Research Hub’s record and data keeping requirements. </li>\n<li>To maintain up-to-date protocols, SOPs and Risk Assessment for all work undertaken </li>\n</ul>\n\n\n  Generic\n\n\n<ul>\n<li>\nTo carry out research on defined, funded projects \n</li>\n<li>\nTo develop and refine experimental strategies \n</li>\n<li>\nTo ensure the validity and reliability of data at all times \n</li>\n<li>\nTo analyse and summarise research results and write reports and manuscripts \n</li>\n<li>\nTo discuss experimental details, research projects and new articles with lab members \n</li>\n<li>\nTo be involved in weekly laboratory meetings, support and advise other projects in the lab \n</li>\n<li>\nTo attend research seminars and other meetings, both internal and external, as required \n</li>\n<li>\nTo present research results at meetings and conferences \nThe above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.\n</li>\n</ul>\n\n\nSkills, knowledge, and experience \n\n\nEssential criteria\n\n\n1.       Masters degree and/or PhD awarded in relevant discipline related to Molecular &amp; Cellular Biology or Immunology\n\n\n2.       A keen interest and background in translational cancer research.\n\n\n3.       Knowledge of cancer biology, particularly the tumour microenvironment \n\n\n4.       Experience in tumour 3D culture/co-culture systems (tumour cell spheroid and/or organoid culture) and their treatment with chemotherapeutics and targeted agents.\n\n\n5.       Expertise in nucleic acid isolation, RT-PCR and quantitative PCR.\n\n\n6.       Experience in SDS-PAGE/western blotting and related techniques such as cell lysate preparation and determination of protein concentration.\n\n\n7.       Experience in advanced light microscopy techniques e.g. multiphoton imaging, confocal imaging, light-sheet imaging.\n\n\n8.       Previous publications, abstracts and presentations at national or international meetings\n\n\n \n\n\nDesirable criteria\n\n\n1.       Experience with and knowledge of advanced molecular biology techniques such as quantitative PCR, Nanopore sequencing, and TapeStation analysis\n\n\n2.       Experience in isolation and analysis of extracellular vesicles, in particular cancer exosomes.\n\n\n3.       Experience with CRISPR/Cas9 technology, including the CRISPR activation (CRISPRa) technique.\n\n\n \n\n\nPlease note that these are PhD level roles but candidates who have submitted their thesis and are awaiting award of their PhDs will be considered. In these circumstances the appointment will be made at Grade 5, spine point 30 with the title of Research Assistant. Upon confirmation of the award of the PhD, the job title will become Research Associate and the salary will increase to Grade 6.\n\n\n \n\n\nThe posts are subject to Disclosure and Barring Service and Occupational Health clearance.\n\n\n","pdfUrl1":null,"location":"London (Central), London (Greater) (GB)","location1":"유럽","location2":null,"location3":null,"type":"2","applyUrl":"https://jobs.kcl.ac.uk/gb/en/job/040751/Research-Associate-Molecular-Biologist","fileUrl1":null,"fileUrl2":null,"fileUrl3":null,"fileUrl4":null,"fileUrl5":null,"fileUrl6":null,"fileUrl7":null,"workingHour":"Full Time","contractType":"Fixed Term","salary":"£38,826 - £43,239 per annum, including London","original_id":"UNI|280105","original_domain":"timeshighereducation.com","original_url":"https://www.timeshighereducation.com/unijobs/listing/280105","original_isPremium":null,"closeType":1,"closedAt":"2022-03-02T00:00:00.000Z","requireEducation":10,"managerTel":"","managerFax":null,"managerEmail":null,"managerName":"KINGS COLLEGE LONDON","managerPhone":null,"managerAddress":"London (Central), London (Greater) (GB)","log_hit":3,"log_like":0,"publishState":1,"organizationName":null,"organizationName_en":null,"organizationName_alt":null,"del_yn":"N","createdAt":"2022-02-03T19:46:23.000Z","updatedAt":"2022-02-03T19:46:23.000Z"},"organization":{"organizationId":494,"lv1Id":null,"lv2Id":null,"name":"King’s College London","name_en":"KINGS COLLEGE LONDON","name_alt":",King’s College London,KINGS COLLEGE LONDON,","introduction":"King’s is a leading university providing world-class teaching and cutting-edge research. We are ranked sixth in the UK for the quality and quantity of research activity (Research Excellence Framework 2014). King’s has played a major role in many of the advances that shape modern life, such as the discovery of the structure of DNA and research that led to the development of radio, television, mobile phones and radar. King’s continues to lead the way on research and pioneers new ideas to make the world a better place.&nbsp;King’s is London’s most central university, with five campuses linked through the heart of the capital, enabling us to build partnerships with key London institutions and form international conversations. We collaborate on research projects, share teaching resources and create internship opportunities to enhance our students’ experience. Current partners include the British Library, British Museum, National Gallery, Shakespeare’s Globe, Southbank Centre, Tate Modern, and more. Our central location and varied partnerships allow students to get connected in one of the world’s most dynamic cities.King’s is not only an enriching experience from an academic perspective – we have a diverse and active student community, with almost 30,000 students from some 150 countries worldwide.","logoImg":"","logoImgSquare":"https://s3.ap-northeast-2.amazonaws.com/jobs.ac.kr/dev/images/fdoip_897356079989.png","homepageUrl":"https://www.kcl.ac.uk/study","infoUrl":"https://www.timeshighereducation.com/world-university-rankings/kings-college-london","country":"GB","state":"London","location":"유럽","address":"Strand, London, WC2R 2LS, United Kingdom","phoneNumber":"+44 20 7836 5454","faxNumber":"","type":1,"univType1":"대학","univType2":"대학교","univType3":"","univType4":"본교","establishedAt":"1829-01-05T00:00:00.000Z","count_majors":null,"count_students":null,"meta_competitiveness":"","publishState":1,"updatedAt":"2021-03-23T10:36:30.000Z","createdAt":"2020-03-04T20:13:07.000Z","default_1_bannerImage":null,"default_1_bannerImageMobile":null,"default_2_bannerImage":null,"default_2_bannerImageMobile":null,"default_3_bannerImage":"https://s3.ap-northeast-2.amazonaws.com/jobs.ac.kr/production/images/1659579746649/King’s College London.jpg","default_3_bannerImageMobile":null}}