{"job":{"jobId":640,"fk_accountId":null,"fk_organizationId":544,"fk_adminAccountId":null,"title":"Research Associate in Protein Design in the Cell","description":"\n<p><strong>Research Associate in Protein Design in the Cell </strong></p>\n<p><strong>Job number</strong> ACAD104411\n<strong>Division/School</strong> School of Chemistry\n<strong>Contract type</strong> Open Ended\n<strong>Working pattern</strong> Full time\n<strong>Salary</strong> £33,797 - £38,017 per annum\n<strong>Closing date for applications</strong> 27-Feb-2020 </p>\n<p>A post-doctoral position is available to develop functional <em>de novo </em>protein designs that operate in living cells. </p>\n<p>This BBSRC-funded post is available for 2 years. The position is between the protein design laboratory of Prof Dek Woolfson (Chemistry and Biochemistry) and the bacterial cell biology laboratory of Prof Nigel Savery (Biochemistry). See: Smith<em> et al.</em> (2019) <em>ACS Synth Biol</em> <em>8</em> 1284. DOI: 10.1021/acssynbio.8b00501.</p>\n<p>Specifically, the work will be to develop <em>de novo </em>protein-protein interactions (PPIs) that operate in <em>E. coli</em> and mammalian cells and orthogonally to the endogenous proteomes and interactomes. The project will build on Woolfson’s set of <em>de novo </em>coiled-coil domains (Fletcher<em>, et al.</em> (2012), <em>ACS Synth Biol</em> 1, 240. DOI:10.1021/Sb300028q; Thomas<em>, et al.</em> (2013), <em>J Am Chem Soc</em> 135, 5161. DOI: 10.1021/Ja312310g; Rhys,<em> et al.</em> (2019) <em>J Am Chem Soc</em> <em>141</em> 8787. DOI: 10.1021/jacs.8b13354) and Savery’s expertise in transcriptional control (Annunziata, <em>et al</em>. (2017), <em>ACS Synth Biol </em>6, 1816. DOI: <a data-mz=\"\" href=\"https://doi.org/10.1021/acssynbio.7b00109\" rel=\"no-follow\">10.1021/acssynbio.7b00109</a>; Haines, et al. (2014) <em>PNAS</em> 111, 4037. DOI: <a data-mz=\"\" href=\"https://doi.org/10.1073/pnas.1322350111\" rel=\"no-follow\">10.1073/pnas.1322350111</a>). The aim is to build <em>de novo </em>PPIs that can activate and repress targeted genes with tight control. A further aim, which is in collaboration with AstraZeneca, is to make these <em>de novo</em> PPIs that respond to small-molecule drugs and, thus, to control transcription by switching the PPIs on and off with the small molecules.</p>\n<p>The position would be best suited to a talented and ambitious early career researcher with an interest in applying <em>de novo </em>protein design in synthetic biology. Essential skills for this role include: experience with the <em>de novo </em>design, synthesis and structural characterisation of synthetic peptides; the design, construction and expression of synthetic genes in <em>E. coli</em>; and biochemical and biophysical whole-cell assays such as FACS. Experience in the design and screening of gene libraries would be an advantage, as would a knowledge mammalian cell biology; however, neither of these are essential for applicants at this stage.</p>\n<p>For informal enquiries, please contact: d.n.woolfson@bristol.ac.uk</p>\n<p>We welcome applications from all members of our community and are particularly encouraging those from diverse groups, such as members of the LGBT+ and BAME communities, to join us.</p>\n","pdfUrl1":null,"location":"Bristol (City Centre), City of Bristol (GB)","location1":"유럽","location2":null,"location3":null,"type":"2","applyUrl":"https://emea3.recruitmentplatform.com/appproc/index.cfm?event=createSessionAfterSessionClear&bSessionClear=true&ID=Q50FK026203F3VBQBV7V77V83&jobboard=0&nPTID=181499&nProfileIdFoundInAGP=&sType=THES&utm_source=THES&utm_medium=paid&utm_campaign=THES","fileUrl1":"","fileUrl2":"","fileUrl3":"","fileUrl4":"","fileUrl5":null,"fileUrl6":null,"fileUrl7":null,"workingHour":"Full Time","contractType":"Fixed Term","salary":"£33,797 - £38,017 per annum","original_id":"UNI|194605","original_domain":"https://www.timeshighereducation.com/unijobs","original_url":"https://www.timeshighereducation.com/unijobs/listing/194605/research-associate-in-protein-design-in-the-cell-/?LinkSource=PremiumListing","original_isPremium":"true","closeType":1,"closedAt":"2020-02-27T00:00:00.000Z","requireEducation":10,"managerTel":"","managerFax":"","managerEmail":"","managerName":"UNIVERSITY OF BRISTOL","managerPhone":"","managerAddress":null,"log_hit":5,"log_like":0,"publishState":1,"organizationName":null,"organizationName_en":null,"organizationName_alt":null,"createdAt":"2020-01-28T09:00:00.000Z","updatedAt":null},"organization":{"organizationId":544,"lv1Id":null,"lv2Id":null,"name":"University of Bristol","name_en":"University of Bristol","name_alt":",","introduction":"<p>Founded in 1876, the University of Bristol combines academic excellence with innovation. Students can choose from more than 500 courses at all levels, from foundation degrees to PhDs.</p><p> </p><p>Ranked joint fifth in the UK for research,* the University is home to more than 25,000 students from across 150 countries. The main campus is at the heart of the historic but vibrant city of Bristol, just a two-hour journey from London. With its diverse culture, independent spirit and acres of green space, the city was named the UK’s best place to live by the <i>Sunday Times</i> in 2017.</p><p> </p><p>Bristol graduates are influential in academia, policy and practice across the world. The University’s alumni network includes leading figures in science, industry, politics and the arts.</p><p> </p>","logoImg":"","logoImgSquare":"https://s3.ap-northeast-2.amazonaws.com/jobs.ac.kr/dev/images/bristol_725531969443.jpg","homepageUrl":"http://www.bristol.ac.uk","infoUrl":"https://www.timeshighereducation.com/world-university-rankings/university-bristol","country":"GB","state":"Bristol","location":"유럽","address":"Senate House, Tyndall Avenue, Bristol, Avon, BS8 1TH, United Kingdom","phoneNumber":"+44 117 928 9000","faxNumber":"","type":1,"univType1":"대학","univType2":"대학교","univType3":"","univType4":"본교","establishedAt":"1875-12-31T00:00:00.000Z","count_majors":null,"count_students":null,"meta_competitiveness":"","publishState":1,"updatedAt":"2020-04-16T14:13:49.000Z","createdAt":"2020-03-04T20:13:07.000Z","default_1_bannerImage":null,"default_1_bannerImageMobile":null,"default_2_bannerImage":null,"default_2_bannerImageMobile":null,"default_3_bannerImage":"https://s3.ap-northeast-2.amazonaws.com/jobs.ac.kr/dev/images/BRISTOL2_1188802760434.jpg","default_3_bannerImageMobile":"https://s3.ap-northeast-2.amazonaws.com/jobs.ac.kr/dev/images/BRISTOL2_759017476913.jpg"}}